Site Directed Crosslinking Studies of the FepA Transport Mechanism
نویسندگان
چکیده
منابع مشابه
Mechanism-Based Studies of the Active Site-Directed Inhibition and Activation of Enzyme Transketolase
Derivatives of phenyl-keto butenoic acids have been reported to be inhibitors of pyruvate decarboxylase, (PDC). The inhibition of transketolase, a thiamine requiring enzyme such as PDF, by meta nitrophenyl derivative of 2-oxo-3-butenoic acid (MNPB) is reported here. These studies indicate that the inhibitor binds to the enzyme at the active site. A two-step inhibition was observed, first th...
متن کاملmechanism-based studies of the active site-directed inhibition and activation of enzyme transketolase
derivatives of phenyl-keto butenoic acids have been reported to be inhibitors of pyruvate decarboxylase, (pdc). the inhibition of transketolase, a thiamine requiring enzyme such as pdf, by meta nitrophenyl derivative of 2-oxo-3-butenoic acid (mnpb) is reported here. these studies indicate that the inhibitor binds to the enzyme at the active site. a two-step inhibition was observed, first the in...
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In total, 59 single Cys-replacement mutants in helix VII and helix X of the lactose permease of Escherichia coli were subjected to site-directed fluorescence labeling in right-side-out membrane vesicles to complete the testing of Cys accessibility or reactivity. For both helices, accessibility/reactivity is relatively low at the level of the sugar-binding site where the helices are tightly pack...
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چکیده ندارد.
15 صفحه اولInsight into the mechanism of phosphoenolpyruvate mutase catalysis derived from site-directed mutagenesis studies of active site residues.
PEP mutase catalyzes the conversion of phosphoenolpyruvate (PEP) to phosphonopyruvate in biosynthetic pathways leading to phosphonate secondary metabolites. A recent X-ray structure [Huang, K., Li, Z., Jia, Y., Dunaway-Mariano, D., and Herzberg, O. (1999) Structure (in press)] of the Mytilus edulis enzyme complexed with the Mg(II) cofactor and oxalate inhibitor reveals an alpha/beta-barrel back...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2012
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2011.11.1363